Figure 12.

Cocoa treatment impaired the mitochondrial-initiated cell death pathway in auditory senescence cells. The Bcl-xL (A), Bax (B) and cytochrome c (C) levels were measured by a colorimetric assay in cell lysates of HEI-OC1, OC-k3, and SV-k1 cells post-treatments. Experimental groups: CTR group (0; 0.5 and 5 μg/mL of CPE) and H₂O₂ group (induced cellular senescence with 100 µM for 1 h in all groups: 0; 0.5 and 5 μg/mL of CPE). The values represent the mean ± SD ng/mL or pg/mL in triplicate. * p < 0.05 vs. CTR group; # p < 0.05 vs. H₂O₂ group (n = 4).