TABLE 1.

Recombinant plasmids used in this work

Plasmids	Relevant characteristics	Source or reference
pC1	pVK102 containing a 20-kb chromosomal fragment from Azotobacter sp. FA8 inserted at the XhoI site	This work
pC1B	a deletion derivative of pC1, obtained by self-ligation after restriction with BamHI	This work
pAC3	pBluescript containing a 3-kb BamHI fragment from pC1	This work
pAC6	pBluescript containing a 6-kb BamHI fragment from pC1	This work
pATpoliK	pAT18 (21) containing a 578-bp amplification fragment from the Azotobacter sp. FA8 phbC gene, interrupted by a kanamycin cassette	This work
pRKpolC1	pRK404 (3) containing a 2.3-kb fragment from pAC6 containing the phbC coding region downstream of lac promoter	This work
pHP1014::2000	Tcr CmrphaC1PaphaDPaphaC2Pa ORF1 ORF2 ORF3 ORF4′	21