TABLE 2.
Analysis of polymer production from different carbon sources in Azotobacter sp. FA8 and several recombinant strainsa
| Strain | Plasmid | Carbon source(s) (% concn) | PHA produced | PHB content (% [wt/wt] of CDW)b |
|---|---|---|---|---|
| Azotobacter sp. FA8 | None | Glucose (3) | PHB | 38 |
| Glucose (3) + octanoate (0.125) | PHB | 26 | ||
| Glucose (0.5) | PHB | 4.5 | ||
| Glucose (0.5) + octanoate (0.125) | PHB | 3.5 | ||
| Azotobacter sp. UBA 60-3 | None | Glucose (3) | NDc | ND |
| Azotobacter sp. UBA 60-3 | pC1 | Glucose (3) | PHB | 25 |
| Glucose (3) + octanoate (0.125) | PHB | 14 | ||
| Azotobacter sp. UBA 60-3 | pHP1014::2000 | Glucose (3) | ND | ND |
| Glucose (3) + octanoate (0.125) | ND | ND | ||
| Azotobacter sp. UBA 60-3 | pRK404 | Glucose (3) | ND | ND |
| Azotobacter sp. UBA 60-3 | pRKpolC1 | Glucose (3) | PHB | 28.5 |
| R. eutropha PHB-4 | None | Glucose (1.5) | ND | ND |
| R. eutropha PHB-4 | pRKpolC1 | Glucose (1.5) | PHB | 4 |
| Octanoate (0.1) | PHB | 1.9 | ||
| Octanoate (0.25) | PHB | 3.9 | ||
| Octanoate (0.5) | PHB | 5 |
a
Azotobacter strains were grown for 24 h in Burk's medium supplemented with carbon sources as indicated. Ralstonia eutropha recombinants were grown for 48 h in mineral salts medium supplemented with carbon sources as indicated.
b
CDW = cell dry weight.
c
ND, not detected (≤1% [wt/wt] of CDW).