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. 2022 Jul 28;93(4):862–869. doi: 10.1038/s41390-022-02219-0

Fig. 3. NET inhibition/degradation does not alter peritoneal leukocyte accumulation, but NET inhibition does decrease bacterial killing in experimental neonatal infectious peritonitis.

Fig. 3

Seven to 10-day old, outbred Swiss-Webster mice were treated with vehicle, nNIF-SCR (10 mg/kg), nNIF (10 mg/kg), Cl-amidine (40 mg/kg), DNase I (25 mg/kg), or meropenem (25 mg/kg) 1 h prior and 4 h after cecal slurry injection (1.3 mg/gram mouse weight) to induce infectious peritonitis. a, b Twenty-four hours after cecal slurry injection, peritoneal lavage fluid was collected and total leukocyte (a) as well as neutrophil (b) recruitment was analyzed by flow cytometry. c Twenty-four hours after cecal slurry injection, peritoneal lavage fluid was collected, diluted 1:10,000 with PBS, grown on an LB agar plate after which colony forming unit (CFU) number was determined via serial dilution and manual counting. Statistical analysis was performed by comparing treatment groups with their respective controls, i.e., nNIF was compared with nNIF-SCR;the other treatment groups were compared to the vehicle control. N = 4–15 mice per group; Mean ± SD. *P < 0.05; **P < 0.01.