Fig. 4.
Glyphosate exposure increases Aβ40-42 and increase cell death in vitro. A Primary cortical neurons were derived from APP/PS1 pups and incubated. Glyphosate was introduced at dosages of 0 µg/mL (vehicle), 10 µg/mL, 20 µg/mL and 40 µg/mL. After 24 h of glyphosate incubation, media was collected, and cell viability was examined. B Soluble Aβ40 levels are increased in the 40 µg/mL (p < 0.0001) and 20 µg/mL (p = 0.001) glyphosate treated groups compared to the 0 µg/mL (vehicle) group. C Soluble Aβ42 levels are increased in a dose-dependent manner in the 40 µg/mL (p < 0.0001), 20 µg/mL (p = 0.001), and 10 µg/mL (p = 0.0092) glyphosate treated groups compared to compared to the 0 µg/mL (vehicle) group. D The 40 µg/mL group had a reduced cell viability when compared to the 20 µg/mL (p = 0.0007), 10 µg/mL (p < 0.0001), and the 0 µg/mL (p < 0.0001) group. Scatterplots with bar graphs are means ± SE. *p < .05 **p < .01, ***p < .001