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. Author manuscript; available in PMC: 2022 Jul 28.
Published in final edited form as: Nat Protoc. 2021 Nov 12;16(12):5592–5615. doi: 10.1038/s41596-021-00626-x

Figure 4. GUIDE-seq example output.

Figure 4.

GUIDE-seq on-target and off-target detailed visualizations are shown from previously published experiments7 (a) low-specificity gRNA targeting AAVS1 site 4, (b) high-specificity gRNA targeting PTPN6 site 2. Each row represents an on-target or off-target site and GUIDE-seq read counts corresponding to that site are shown to the right. A dot represents matches with respect to the intended target sequence, and colored nucleotides indicate the sequence of mismatches. Visualizations are ordered by GUIDE-seq read counts. Manhattan plots for (c) AAVS1 site 4 and (d) PTPN6 corresponding to the same GUIDE-seq data are shown; x-axis represents chromosomal location and y-axis represents GUIDE-seq read counts. Original figure.