| Plus PCR1 | Minus PCR1 | ||
|---|---|---|---|
| Component | Volume (μl) | Component | Volume (μl) |
| Nuclease-free water | 11.9 | Nuclease-free water | 11.9 |
| Platinum Taq PCR Rxn Buffer (−MgCl2) (10X) | 3 | Platinum Taq PCR Rxn Buffer (−MgCl2) (10X) | 3 |
| dNTP mix (10 mM) | 0.6 | dNTP mix (10 mM) | 0.6 |
| MgCl2 (50 mM) | 1.2 | MgCl2 (50 mM) | 1.2 |
| oGS201 primer (10μM) | 1 | oGS202 primer (10μM) | 1 |
| TMAC (500 mM) | 1.5 | TMAC (500 mM) | 1.5 |
| oGS200 primer (10 μM) | 0.5 | oGS200 primer (10 μM) | 0.5 |
| Platinum Taq polymerase | 0.3 | Platinum Taq polymerase | 0.3 |
| Template DNA (from step 26) | 10 | Template DNA (from step 26) | 10 |
| Total | 30 | 30 | |
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