| Step | Temperature | Time |
|---|---|---|
| Denaturation | 98°C | 5 min |
| 98°C | 15 s | |
| 35 cycles | 68°C | 20 s |
| 72°C | 30 s | |
| Extension | 72°C | 5 min |
| End | 4°C | Hold |
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