Table 4.

Truncation (grey) and elongation (red) peptide length contribute to VPAC1R/VPAC2R-selective peptide analogues.

C-Terminal Truncation/Elongation
	1				5				10					15					20					25					30					35
PACAP38	H	S	D	G	I	F	T	D	S	Y	S	R	Y	R	K	Q	M	A	V	K	K	Y	L	A	A	V	L	G	K	R	Y	K	Q	R	V	K	N	K
[R16]-PACAP(1–23)	H	S	D	G	I	F	T	D	S	Y	S	R	Y	R	R	Q	M	A	V	K	K	Y	L	A	A	V	L	G	K	R	Y	K	Q	R	V	K	N	K
VIP	H	S	D	A	V	F	T	D	N	Y	T	R	L	R	K	Q	M	A	V	K	K	Y	L	N	S	I	L	N
RO 25-1553 1	H	S	D	A	V	F	T	E	N	Y	T	K	L	R	K	Q	L	A	A	K	K	Y	L	N	D	L	K	K	G	G	T
PG 96-249a 2	H	S	D	A	V	F	T	E	N	Y	T	K	L	R	K	Q	L	A	A	K	K	Y	L	N	D	L	K	K	G	G	T

¹ The following non-canonical amino acid modifications observed in RO 25-1553: Ac-His¹ and Nle¹⁷. Lactam bridge formed between Lys²¹ and Asp²³ [147]. ² The following non-canonical amino acid modifications observed in PG 96-249a: Ac-His¹ and Nle¹⁷ [147].