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. 2022 Jul 25;23(15):8188. doi: 10.3390/ijms23158188

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Printed spheroids in CANAHA bioink. (A) Time course of A549-BFP spheroid culture (yellow arrows indicate outgrowth of cells; space in yellow dashed lines indicates printed gel), scale bar 1000 µm. (B) Fluorescence microscopy image of A549-BFP spheroid 7 d after printing, scale bar 100 µm. (C) Rate of printed spheroids, n = 7 prints with 780 spheroids in total. (D) Calcein-AM and ethidium-HD-1 staining (live and dead assay) of bioprinted A549 spheroids 7 d after printing, scale bar 100 µm.