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. 2022 Jul 25;23(15):8188. doi: 10.3390/ijms23158188

Table 1.

Cultured cell lines and culture conditions. FBS: fetal bovine serum, P/S: penicillin/streptomycin, FGF2: fibroblast growth factor 2, BFP: blue fluorescent protein, RFP: red fluorescent protein, GFP: green fluorescent protein.

Cell Line (Label) Culture Medium Additive Nr. of Cells
Hepa1-6 DMEM 10% FBS 5000
MCF7 DMEM 10% FBS 5000
A549 (BFP) DMEM 10% FBS 5/10,000
A549 (BFP), HPMEC (RFP), MSC (GFP) DMEM,
ECGM,
alpha-MEM
10% FBS, 100 U/mL P/S
5% FBS, 50 U/mL P/S
10% FBS, 5 ng/mL FGF2, 1% P/S
25,000
(5:3:2 cell and medium ratio)