TABLE 1.

Oligonucleotide primers used in this study

Primer	Sequence (5′ to 3′)	Amino acid sequence	Application
nas22	TGYCCNTAYTGYGGNGT	CPYCG	nasA/narB PCR amplification
nas964	CARCCNAAYGCNATGGG	QPNAM	nasA/narB PCR amplification
nasA1735	ATNGTRTGCCAYTGRTC	DQWHT	nasA PCR amplification
nas1933	CARTGCATNGGNAYRAA	F/L V/I/M PMH	nasA/narB PCR amplification
fd1	AGAGTTTGATCCTGGCTCAG		16S rRNA forward primer
rp2	ACGGCTACCTTGTTACGACTT		16S rRNA reverse primer
M13F	GTAAAACGACGGCCAG		Forward sequencing primer, M13 vector
522F	CAGCCGCGGTAATAC		Forward sequencing primer, 16S rRNA
1056F	TGGCTGTCGTCAGCTCGTGT		Forward sequencing primer, 16S rRNA
M13R	CAGGAAACAGCTATGAC		Reverse sequencing primer, M13 vector
1056R	ACACGAGCTGACGACAGCCA		Reverse sequencing primer, 16S rRNA
522R	GTATTACCGCGGCTG		Reverse sequencing primer, 16S rRNA