Skip to main content
. 2021 Aug 27;39(3):673–693. doi: 10.5114/biolsport.2022.107018

TABLE 3.

Types of methodology used to collect saliva samples in the included articles.

Study Salivary markers Stimulated Yes / No Mouth rinse Yes / No Dietary restriction time Collection type Time of the collection Manufacturer of reagents
Long-term effect of basketball training periods on salivary marker levels
Andre et al. 2018 [26] Cortisol
Testosterone
No Yes 60 min Swabbing 1:00–3:00pm Salimetrics
Arruda et al. 2013 [23] Cortisol No No Overnight fasting n/a 7:30am DSL
Atalag et al. 2019 [32] Cortisol No No 60 min Passive drooling 10:00am–12:00pm Salimetrics
Gonzalez-Bono et al. 2002 [51] Cortisol
Testosterone
No No Overnight fasting Passive drooling 8:30–9:00am OD (C) ICN (T)
He et al. 2010 [25] Cortisol
Immunoglobulin A
Total protein
Lactoferrin
No Yes n/a Spitting 5:30–6:30pm DRG (C)
Sigma (IgA)
Calbiochem (LF)
Moreira et al.2011 [40] Cortisol
Immunoglobulin A
No No 60–90 min Passive drooling n/a (in the afternoon, same time of the day) ALPCO
Nunes et al. 2011(a) [15] Cortisol
Testosterone
Immunoglobulin A
No No n/a Passive drooling 7:30am; 9:30am; 11:00am; 5:30pm Salimetrics (T; IgA) DSL (C)
Nunes et al. 2014 [18] Cortisol
Testosterone
Immunoglobulin A
No Yes 90 min Passive drooling 7:30am; 9:30am; 12:00pm; 6:00pm Salimetrics
Miloski et al. 2015 [36] Testosterone No No 120 min Passive drooling 3:00pm Salimetrics
Azarbayjani et al. 2011 [21] Immunoglobulin A
Total protein
No Yes n/a n/a n/a (pre-, post- and 1h post- completion of exercise) Bradford
Moraes et al. 2017 [22] Immunoglobulin A No No 120 min Passive drooling 3:00pm Salimetrics
Moreira et al. 2008 [42] Immunoglobulin A
Total protein
No Yes 120 min Passive drooling 3:30pm ALPCO (IgA)
Pierce (TP)
Short-term effect of basketball training periods on salivary marker levels
Moreira et al. 2018 [19] Cortisol
Testosterone
Alpha-amylase
No No n/a n/a n/a (pre- control and experimental conditions; post- both conditions; post- SSGs) Salimetrics
Nunes et al. 2011(b) [41] Cortisol
Testosterone
Immunoglobulin A
No No n/a n/a 7:30am; 9:30am; 11:00am; 5:30pm Salimetrics
Sansone et al. 2018 [16] Cortisol
Testosterone
No No n/a n/a n/a (pre- warm-up, and 15min post- SSGs. Experimental sessions started at 5:00pm) LDN

Note: n/a – not available, not provided in article; SSGs – small-sided games; Salimetrics – Salimetrics LLC, Carlsbad, CA, USA; DSL – Diagnostic Systems Laboratories, INC, Webster, TX, USA; ICN – ICN Biomedicals, Costa Mesa, CA, USA; OD – Orion Diagnostica, Espoo, Finland; DRG – DRG Diagnostics, Marburg, Germany; Sigma – Sigma-Aldrich, Poole, UK; Calbiochem – Calbiochem, Darmstadt, Germany; ALPCO – ALPCO diagnostics, Salem, MA, USA; Bradford – Bradford Solution for Protein Determination; Pierce – Pierce Biotechnology, Rockford, IL, USA; LDN – Labor Diagnostika Nord, Germany; C – cortisol; T – testosterone; IgA – immunoglobulin A; AA – alpha-amylase; TP – total protein; LF – lactoferrin.