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. 2022 Jul 26;11(15):2298. doi: 10.3390/cells11152298

Figure 3.

Figure 3

Effects of DMSO on matrix remodeling and cell organization. (a) ECM deposition was detected by reticulin staining on HepaRG cells differentiated from D15 of culture in the presence (D30+ DMSO) or the absence of DMSO (D30 − DMSO). (b) Microarray data for TIMP1, TIMP2, MMP7, MMP19, and MMP24 are expressed relative to conditions D30 − DMSO, arbitrary set to 1. The significance of DMSO treatment (D30 + DMSO vs D30 − DMSO) was evaluated by an unpaired t-test * p < 0.05, ns: not significant. (c) Immunostaining of fibronectin, laminin, collagen type I, III, and IV in HepaRG cells differentiated in the presence of DMSO (D30+ DMSO). Immunolocalization without primary antibody was performed as control. (d) Immunolocalization of F-actin in HepaRG cells differentiated from D15 of culture in the presence (D30+ DMSO, left panel) or in the absence of DMSO (D30 − DMSO, right panel). Nuclei were visualized by staining with Hoechst (shown in blue). Scale bars = 7 µm.