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API treatment of KC-HPC mice increased CD40 expression on M1 TAM and iNOS in the tumor. (A,B). Flow cytometric analysis of CD40 expression on M1 TAM (CD11b+Ly6C+/−Ly6G−F4/80+CD206−MHCII+) in the tumors of KC-HPC and KC-HPC-API mice. (C,D) WB analysis and representative quantification of iNOS protein in the tumors from KC-HPC and KC-HPC-API mice. Data are presented as the mean ± S.D. of KC-HPC (n = 4), KC-HPC-API (n = 4) mice. * p < 0.05 (by two-tailed t test).
