Fig. 1.

Fasting activates the HPA axis: Body weight change (A), plasma leptin (B) and glucose (C) levels, ARC gene expression (D), Crf gene expression in the PVN (E), plasma corticosterone levels (F), FosB (G) and double FosB/tdTomato (H) immunostaining in the PVN in fed and 36 h-fasted adult (8–10 weeks) male mice (n = 8–10) euthanized at 10am as described in the Additional file 1: Fig. S1. Data from graphs A to F were obtained in WT animals, while graphs G and H and the photomicrographs (×20, insert ×10) in I were obtained from Crh-IRES-Cre::tdTomato (red) mouse and submitted to immunostaining for FosB (green). Data are expressed as mean ± SEM and were analyzed by unpaired t-test or Mann-Whitey. *p < 0.05 when comparing fasting vs fed mice