Fig. 2.

Detection of ApoE in ZIKV-induced ER membrane rearrangements in infected Huh7.5 cells. a Observation, by confocal microscopy, of the ApoE-Zika E protein channel with the ER marker calnexin in infected Huh7.5 cells and Pearson coefficient graph obtained from 30 z-stacks. Scale bars = 8 µm. b TEM observations of the characteristic membrane rearrangements induced by ZIKV infection in infected Huh7.5 cells. Thin black arrows: Zippered ER; thin white arrows: viral particles; bold black arrows: vesicle packets; asterisk: cytoplasmic vacuolization. Scale bars = 500 nm. c Immunogold labeling, on cryo-TEM sections, of ApoE and Zika E protein with 6 nm and 12 nm gold-conjugated Abs, respectively, in infected Huh7.5 cells. Insets show a high magnification of the area indicated by the thin arrow. Scale bars = 200 nm