Figure 4.

Cytokine profiles in the tumor microenvironment of mice receiving intraperitoneal injections of free or conducting oxRilac. (a) Schematic overview of samples obtained for cytokine analysis; (b) WPGMA-weighted hierarchical clustering calculated from z-scored intratumoral cytokine concentrations; (c) Spearman’s correlation between intra- and extratumoral (lavage) cytokine concentrations; (d) principal component analysis (PCA) calculated from z-scored intratumoral cytokine concentrations of individual animals showing PC scores and loadings; (e) absolute intratumoral cytokine concentrations. Bar graphs show medians and individual values. The limit of detection is indicated as a dashed red line. Statistical analysis was conducted using one-way analysis of variance (ANOVA) and Tukey’s post hoc testing (* p < 0.05, ** p < 0.01, *** p < 0.001). ns = nonsignificant; U = untreated; F = free mode; C = conducting mode.