Figure 3.

Compressive forces and TLR4 blocking antibody modulate TLR4 production and MyD88 in primary PDL cells. (A) Protein levels of TLR4 and MyD88 were determined by Western blot in different conditions: HMGB1 (100 ng/mL), TLR4 blocking antibody (5 µg/mL) (TLR4: TLR4 blocking antibody) and compressive force (CF) 2 g/cm² for 3 and 24 h. Three different donors showed similar patterns with reduction of TLR4 antibody. TLR4 production was upregulated under 3 h compression forces. MyD88 production was reduced in all conditions. (B) Quantification of three donors, normalized to the control with stain-free technology. Data were tested for normal distribution by Shapiro–Wilk test. Afterward, a one-way analysis of variance (ANOVA) followed by Tukeys’ post hoc test was performed.