Skip to main content
. 2022 Jul 23;23(15):8139. doi: 10.3390/ijms23158139

Figure 4.

Figure 4

Functional expression of T. vaginalis ENT3 in SUPKO. (A) 500 nM of [3H]-cytidine was significantly higher after expression of TvagENT3 in SUPKO (1.8 × 10−4 ± 1.3 × 10−5 vs. 1.5 × 10−5 ± 9 × 10−6 pmol(107 cells)−1s−1, respectively; p < 0.0001). The cytidine uptake in SUPKO was not significantly different from zero (p = 0.18). (B) Uptake of 50 nM [3H]-adenosine was 94.6% lower in SUPKO than in the same cells expressing TvagENT3 (0.00060 ± 0.00007 vs. 0.0080 ± 0.0004 pmol(107 cells)−1s−1; p < 0.0001). Both slopes were significantly different from zero (p = 0.004 and 0.0003, respectively). (C) Uptake of 50 nM [3H]-adenosine in SUPKO over 60 s in the presence of variable concentrations of unlabelled adenosine and inosine. Inset: Conversion of adenosine inhibition data to a Michaelis–Menten saturation plot.