Figure 3.
Phenotypes and P. brassicae DNA content in the roots of rapeseed treated with Hz36 and Hk37 strains. (A) Phenotype of rapeseed infected by P. brassicae alone or together with Hz36 for 30 days. (B) Disease index of samples in A. (C) P. brassicae DNA content in the samples of A detected by qPCR. (D) qPCR analysis was performed to assess the expression levels of P. brassicae at 21 days after inoculation in the sample of A. Three plant roots were taken as a mixed sample. (E) Phenotype of rapeseed infected by P. brassicae alone or together with Hk37 for 30 days. (F) Disease index of samples in E. (G) P. brassicae DNA content in the samples of E detected by qPCR. Water indicates the plants inoculated with water as a negative control; PB represents the plants inoculated with P. brassicae as a positive control; PB+Hz36 indicates the plants co-inoculated with P. brassicae and strain Hz36; PB+HK37 represents the plants co-inoculated with P. brassicae and strain HK37, scale bar = 1 cm. The student’s two-tailed t-test was performed for comparison of means between two data points in (C,D,G,H), and the data are shown as mean ± s.d. (n = 3 biological replicates, 3 plant roots were taken as a mixed sample), black dots show three biological replicates. Exact p-values for all comparisons are shown in the source data. Experiments were repeated three times with similar results.