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. 2022 Jul 14;12:924354. doi: 10.3389/fonc.2022.924354

Figure 1.

Figure 1

Decitabine increases TAp63 protein and mRNA levels in squamous cells. (A) Representative Western blots of TAp63 in HaCaT, FaDu and SCC-25 cells cultured in the presence of the indicated concentration of decitabine for four days. β-actin is shown as loading control. Densitometry data normalized to β-actin are shown below, indicating fold change ± SEM compared to control cells (0, DMSO only; n = 3 for each dose in each cell line). (B) TAP63 mRNA levels normalized to ACTB mRNA (n = 3 to 5 biological replicates, each with technical triplicates). Data are shown as fold change ± SEM compared to control cells (0, DMSO only). Note the different y axis range for FaDu cells. **p < 0.01; ***p < 0.001 compared to control.