Figure 5.

DNMT1 depletion alters TP63 isoform mRNA levels and decreases KRT5 mRNA levels. (A) Representative Western blots of DNMT1 in two independent clones of HaCaT and FaDu DNMT1-shRNA cells cultured for four days in the absence (–) or presence (+) of 2 µg/ml doxycycline (DOX). β-actin is shown as loading control. Densitometric quantitation of DNMT1 normalized to β-actin is shown below for each clone. (B) Relative changes in the ratio of TAP63 to ΔNP63 mRNA levels without induction (CTR) or after six days of 2 µg/ml doxycycline (DOX). Data were normalized to ACTB mRNA for each clone in each cell line and are shown as the average change for the two independent clones for each cell line. (C) KRT5 mRNA levels in response to DNMT1-shRNA induction. Plots show fold change ± SEM compared to non-induced cells. n = 3 to 5 biological replicates; *p < 0.05; **p < 0.01.