Figure 8.

Immunohistochemical labeling of external segment of rods, rod bipolar cells, and RGCs. From left to right, the different animal models are presented (C57BL/6J; Opn4^−/−; Rd10; Opn4^−/− × Rd10). From top to bottom, the different agents and antibodies used are presented. DAPI: intercalating marker of cell nuclei; Rhodopsin (Rho): opsin located in the outer segments of the rods; PKCα: bipolar cell structural marker; Brn3a: protein located in the ganglion cells of the retina. The bottom row shows “Merged” of different labelings. As can be seen, in the models that present photoreceptor degeneration (rd), the marking of the outer segments of the photoreceptors disappears, while the cells of the inner retina show no apparent differences in any of the four animal models. The scale bar indicates 50 µm.