Fig. 2.

Transcriptomic and gene enrichment analysis upon AT treatment in OA chondrocyte cells. A Heatmap of expression of transcripts from triplicate experiments of OA and OA + AT. B Volcano plot of differential expressed genes. Yellow points represent upregulated genes for log₂FC > 2, and blue points represent upregulated genes for log₂FC < -2. C GSEA analysis of negative regulated genes shows PI3K–AKT–mTOR signaling. D The analysis of KEGG pathway enrichment for negative regulated genes. P13K-AKT-mTOR signaling pathway was enriched from these negative regulated genes. E Waterfall plot of fold change in gene expression in OA chondrocyte cells treated with 0.05 µM AT for 6 h; selected TNFSF11 gene is highlighted