TABLE 1.

Strains and plasmids

Strain or plasmid	Phenotype	Reference or source
lactis
LM2301	Wild type pip, plasmid free	45
BG301	LM2301 with single-copy, chromosomal pip-emm6c	This work
S. gordonii GP1223	Expresses M6c on its surface	8
E. coli
DH5αmcr	Cloning strain	Life Technologies
CC118	phoA strain for screening TnphoA fusions	26
JM110	dam	49
Plasmids
pSMB102	Source of emm6	30
pSMB104	Source of emm6 used for cloning	This work
pSA3	Shuttle vector	9
pRB04	pip cloned in shuttle vector pSA3	15
pUC19	Cloning vector	49
pUCpip	pip cloned in pUC19	This work
pUCpipM6c	pip-emm6c cloned in pUC19	This work
pUCΔpipM6c	Deletion in the pip region of pip-emm6c cloned in pUC19	This work
pTRKH2	High-copy-number shuttle vector	31
pPip	pip with pip promoter in pTRKH2	This work
pTRK568	Promoter P6 in pTRKH2	T. R. Klaenhammer (gift)
pBG568	pTRKH568 with unique XbaI site	This work
pPipM6c	pip-emm6c with pip promoter in pTRKH2	This work
pP6pip	pip with P6 promoter in pBG568	This work
pP6pipM6c	pip-emm6c with P6 promoter in pBG568	This work
pP16pip	pip with P16 promoter in pBG568	This work
pP16pipM6c	pip-emm6c with P16 promoter in pBG568	This work
pΔPipM6c	pPipM6c with large deletion in pip	This work
pΔPipM6c-r	Same as pΔPipM6c except reverse orientation of Δpip-emm6c in pTRKH2	This work
pGhost6	Integration vector used for allelic exchange	25 (Appligene, Pleasanton, Calif.)
pGhPipM6c	pip-emm6c cloned in pGhost6	This work
pPLA16	Source of promoter PLA16	1