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N-glycosylation changes induced by 2DG. (A) Comparison of the three N-glycan types (high-mannose, hybrid, and complex) with and without 2DG treatment. (B) Classification of the 1560 IGPs into 42 groups based on their N-glycan composition. (C–E) Three examples representing intact N-glycopeptides with high-mannose, hybrid, and complex glycan types, respectively. Note: *, **, indicates a p-value < 0.05, 0.01, respectively.
