. 2022 Jul 22;11(8):1092. doi: 10.3390/biology11081092

Table 1.

PCR primers used in the study.

Primer Name	Sequence(5′-3′)	Usage
GSX7-2097F	GGTTACATATGTTTATTACTAAGC	Full-length cloning of forward promoter
GSX7-2097R	GGGTTTGAGGCGACCGGCGACCGGC	Full-length cloning of forward promoter
GSX7R-2097F	GGGTTTGAGGCGACCGGCGACCGGC	Full length cloning of reverse promoter
GSX7R3R	GGTTACATATGTTTATTACTAAGCA	Full length cloning of reverse promoter
GSX7R-1765F	ATGGCGCATGATTATATTCCGATTA	5′ deletion cloning
GSX7R3R	GGTTACATATGTTTATTACTAAGCA	5′ deletion cloning
GSX7R-1198F	GTCTCCGTGGGCTGCGCTAGCTTGC	5′ deletion cloning
GSX7R3R	GGTTACATATGTTTATTACTAAGCA	5′ deletion cloning
GSX7R-554F	ACTTGTACTTAGAAAATAACATGTT	5′ deletion cloning
GSX7R3R	GGTTACATATGTTTATTACTAAGCA	5′ deletion cloning
GSX7R-2097F	GGGTTTGAGGCGACCGGCGACCGGC	3′ deletion cloning
GSX7R-1543R	GGAGATAGACATTATTTGGAAATGA	3′ deletion cloning
GSX7R-2097F	GGGTTTGAGGCGACCGGCGACCGGC	3′ deletion cloning
GSX7R-1212R	CGCGCCTGCCGTTGCCGCCGGTCCT	3′ deletion cloning
hptF	ACACAGCCATCGGTCCAGA	hpt maker gene identification
hptR	TAGGAGGGCGTGGATATGTC	hpt maker gene identification
Os8GSX7F	GCTCGACGCATGCATGGCACAG	RT-PCR
Os8GSX7R	GTCCAATATGTGGAATCTGATC	RT-PCR
GUSF	GAACTGGCAGACTATCCCGCCGG	RT-PCR
GUSR	CCTGCCAGTCAACAGACGCGTGG	RT-PCR
ActinF	CATGCTATCCCTCGTCTCG	Reference gene
ActinR	CGCACTTCATGATGGAGTTG	Reference gene