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. 2022 Jul 25;23(15):8184. doi: 10.3390/ijms23158184

Figure 2.

Figure 2

Depletion of EDEM3 from prostate cancer cells increases expression of ER stress and apoptosis-associated genes. (A,B) mRNA and protein levels of EDEM3 in LNCaP and CWR22Rv1 cells following shRNA-mediated EDEM3 gene knockdown compared with empty vector control. mRNA levels shown as mean ± s.e.m. Protein levels detected by Western blotting. GAPDH was used as a loading control. (C) Volcano plot showing transcriptomic analysis of CWR22Rv1 cells following EDEM3 gene knockdown. Significantly downregulated genes are shown in blue and significantly upregulated genes are shown in red. Significance was determined using an adjusted p value. * p < 0.05, ** p < 0.01. (D) Heatmap showing the log fold change in gene expression levels of ER stress and apoptosis-associated genes from RNA sequencing of CWR22Rv1 cells following EDEM3 knockdown. (E) mRNA expression levels of ER stress-associated genes and pro-apoptotic genes following EDEM3 knockdown in LNCaP cells. n = 3 and data are mean ± s.e.m. (F) Western blot analysis of GRP78 in LNCaP cells following EDEM3 gene knockdown.