Figure 4.

Effects of inhibitors for intracellular signaling factors on SRM and AMD1 mRNA levels. (A,B) Cells were pre-incubated in the absence (Veh) and presence of 10 μM CHIR99021 (CHIR) or 10 μM Naphthol AS-E (Naph) for 0.5 h. Then, the cells were incubated with 0.8 or 10.8 mM MgCl₂ for 6 h. The mRNA levels of SRM and AMD1 were represented as a percentage of 0.8 mM MgCl₂. (B) Cells were pre-incubated in the absence (Veh) and presence of 10 μM U0126 or 10 μM apigenin for 0.5 h. Then, the cells were incubated with 0.8 or 10.8 mM MgCl₂ for 6 h. The mRNA levels of SRM and AMD1 were represented as a percentage of 0.8 mM MgCl₂. n = 3–4. Error bars indicate SEM. ** p < 0.01 significantly different from 0.8 mM MgCl₂. ^## p < 0.01 significantly different from Veh. ^NS p > 0.05.