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. 2022 Jul 26;27(15):4781. doi: 10.3390/molecules27154781

Figure 1.

Figure 1

Characterization of PLP binding to FnYggS. (A) The FnYggS and FnYggS-SeMet solutions were yellow and colorless, respectively. (B) Native FnYggS exhibited an absorption peak at 425 nm due to PLP binding, whereas FnYggS-SeMet did not exhibit an absorption peak, indicating the absence of PLP binding. The absorption peak of PLP was seen at 380 nm. (C) Purified native and SeMet-derivatized FnYggS solutions and size exclusion chromatography results. Both proteins existed as monomers in solution.