Figure 7.

Inhibition of NE attenuated HFD feeding-induced leukocyte infiltration and pro-inflammatory cytokine expression in WAT. C57BL/6 mice (8-week-old males) were fed with either a standard chow diet, an HFD (60% kcal of fat), or HFD plus NEI (GW311616A, 25 mg/kg HFD) for 14 weeks before being perfused with PBS to remove blood cells in the circulation. SVF cells were isolated from eWAT for flow cytometry analyses of total CD45⁺ immune cells (A), CD45⁺CD11b^highF4/80⁺ adipose tissue macrophages (ATMs) (B), and CD45⁺CD11b⁺Ly6G⁺ neutrophils (C). Total RNA samples from eWAT were used for RT-PCR to quantify mRNA expression levels of IL-1β, TNFα, and IL6 (D). The expression levels were normalized against the 36B4 reference gene. All the values are expressed as mean ± SD, n = 5 or 6 in each group of mice. ^# p < 0.05, ^## p < 0.01, chow versus HFD; * p < 0.05, HFD vs. HFD plus NEI. See also Figure S5.