Table 1.
Comparison between commonly used commercially available spatial transcriptional profiling platforms (Adapted from Bassiouni et al. [60]).
| Comparison Parameters | 10× Genomics Visium | NanoString Ge-oMX DSP |
|---|---|---|
| Tissue compatibility | Compatible with fresh frozen tissue | Compatible with fresh frozen tissue and FFPE |
| RNA quality | Needs RNA integrity number >7 | No requirements for RNA quality |
| Tissue preparation | Tissue is mounted on a specialized gene expression slide | Tissue is mounted on a standard microscope slide |
| Tissue size | 6.5 × 6.5 mm per capture area | 14.6 × 36.2 mm |
| Detection area | Within the full capture area | Within user-defined regions of interest |
| Cellular resolution | ~10 cells/feature | ~20–200 cells/region of interest |
| Direct RNA detection | Absent | Present |
| Concurrent protein detection | Present | Present |