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. 2022 Jul 22;44(8):3312–3323. doi: 10.3390/cimb44080228

Figure 6.

Figure 6

(a) Effects of the DOE on the protein expressions of p-AKT, AKT, p-ERK1/2, and ERK1/2 in B16F10 cells. B16F10 cells were treated with 3.91 μg/mL of DOE. Protein expressions of p-AKT, AKT, p-ERK1/2, and ERK1/2 were analyzed by Western blotting. Equal protein loading was confirmed using β-actin antibody. (b) Protein levels of p-AKT, AKT, p-ERK1/2, and ERK1/2. The relative level of each protein was calculated based on the intensity of β-actin protein and CREB. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. 0 h.