Table 1. Information about the primer sequences.
| Genes | Primer sequences (5′–3′) | Anneal temperature (°C) | |
|---|---|---|---|
| Forward | Reverse | ||
| NANOG | AAGTACCTCAGCCTCCAGCA | GTGCTGAGCCCTTCTGAATC | 60 |
| OCT4 | GTGAGAGGCAACCTGGAGAG | TCGTTGCGAATAGTCACTGC | 60 |
| CASP3 | GACGGACAGTGGGACTGAAGA | GCCAGGAATAGTAACCAGGTGC | 60 |
| SIRT1 | ATCGTCACCAATGGTTTCCA | GGATCTGTGCCAATCATGAG | 60 |
| SOX2 | AAGAGAACCCCAAGATGCACAACT | GCTTGGCCTCGTCGATGAAC | 60 |
| ATG5 | GGTTTGAATATGAAGGCACACCA | TGTGATGTTCCAAGGAAGAGCTG | 60 |
| LC3 | AACGAAATTCCTGGTGCCTGA | AAGGCTTGGTTAGCATTGAGCTG | 60 |
| P62 | AAGAGAAGCCGCCTGACAC | CGACTCCAGGGCGATCTTAT | 60 |
| KEAP1 | ACCCAATTTCTGCCCCTGAG | ACTTGACCTGCAGCGTAACA | 60 |
| NRF2 | GCCCAGTCTTCATTGCTCCT | AGTCCTCCCAAACTTGCTC | 60 |
| UCHL1 | ACTTTGGATTCGCTCGGTAC | CGCTTATCTGCAGACCCCAA | 60 |
| GPX1 | CACAACGGTGCGGGACTA | CATTGCGACACACTGGAGAC | 60 |
| NOX2 | TGTATCTGTGTGAGAGGCTGGTG | CGGGACGCTTGACGAAA | 60 |
| SOD1 | TGACTGCTGGCAAAGATGGT | TTTCCACCTCTGCCCAAGTC | 60 |
| GAPDH | TTCCACGGCACAGTCAAG | ATACTCAGCACCAGCATCG | 60 |