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Schematic representation of the construction of the suicide plasmid pK18-3 designed to deliver any gene into the B. lactofermentum chromosome and subcloning of the six-His-tagged xylanase gene (ΔxysA-6His) into the unique NdeI site (NdeI*). For details, see the text. NdeIK and BglIIK, Klenow-filled NdeI and BglII; Pkan, promoter of the kan gene from Tn5; T1, terminator of the methylenomycin resistance gene from Streptomyces coelicolor A3 (2); T2, terminator of phage fd.
