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. 2022 Jul 26;13(8):1338. doi: 10.3390/genes13081338

Figure 6.

Figure 6

BAG3 blocks ox-LDL-induced EndMT through formation of CASA complex with HSP70 and HSPB8 in HUVECs. (A) Co-IP analysis for the interaction of BAG3 with HSP70 or HSPB8. HUVEC extracts were immunoprecipitated with anti-BAG3 antibody and probed with anti-HSP70 or anti-HSPB8 antibody. (n = 3). (B–H) BAG3, HSP70, HSPB8, CD31, and α-SMA protein expressions and the LC3-II/LC3-I ratio were analyzed using Western blotting. Fold changes are shown (n = 5). (I) Morphological changes post the indicated treatments were observed under a microscope (scale bar indicates 100 µm). (J,K) Transwell cell invasion assay was performed to analyze the migration ability of cells (scale bar indicates 100 µm). (n = 3). (L) Representative images of double-fluorescent staining with α-SMA (green) and CD31 (red). The nuclei were stained blue with DAPI (scale bar indicates 50 µm). The data are presented as the mean ± S.E.M. ns p > 0.05, * p < 0.05, ** p < 0.01.