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. 2022 Jul 26;23(15):8203. doi: 10.3390/ijms23158203

Figure 5.

Figure 5

Y2H assay of WA1 with S1 and C1. (A) Schematic diagram of the WA1, C1, and S1 intact proteins and truncated proteins used for assay. The conserved domains of these proteins were shown as in the colored box. (B) WA1 interacted with R2 domain of C1 and N terminus of S11-61. (C) WD repeat units interacts with R2 domain of C1 and N terminus of S11-61. AD, pGADT7 empty vector; BD, pGBKT7 empty vector. SD-LT, SD Base medium +DO Supplement-Leu/-Trp; SD-AHLT, SD Base medium +DO Supplement-Ade/-His/-Leu/-Trp.