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. 2022 Jul 14;13:884006. doi: 10.3389/fimmu.2022.884006

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Copyright © 2022 Jiang, Li, Zhang, Zhou, Guo, Zhou, Ming

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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T-cell subsets in the spleen of mice in each group were analyzed by flow cytometry on day 28 after SEA treatment and on day 7 after skin transplantation. (A) Compared with the control group, on the 28th day after SEA treatment, the percentages of CD4⁺IL-4⁺ T and Treg cells in the spleen of mice in the experimental group were significantly upregulated, while CD4⁺ IFN-γ⁺ and CD4⁺ IL-17⁺ T-cell ratios were not significantly different. *p < 0.05, **p < 0.01, n = 6. (B) On the 7th day after transplantation, the percentages of CD4⁺ IL-4⁺ T and Treg cells in the spleen of mice in the experimental group were still significantly upregulated compared with the control group, and there was no significant difference in the ratio of CD4⁺ IL-17⁺ T cells, but compared with the control group, the percentage of CD4⁺ IFN-γ⁺ T cells was significantly decreased. *p < 0.05, **p < 0.01, n = 6.