TABLE 4.
Effect of different treatments on the viability of A. suum eggs in pure solutiona
| Treatment and step | Reagent
|
% Viability (standard deviation)
|
Tukey groupingb
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| All steps | Hydrophilic extraction (reagent 1) | Lipophilic extraction (reagent 2) | All steps | Minimum exposurec | Maximum exposured | All steps | Minimum exposurec | Maximum exposured | |
| Flotation | |||||||||
| 1 | ZnSO4 | 81.4 (1.4) | A | ||||||
| 2 | MgSO4 | 85.0 (1.0) | A | ||||||
| 3 | NaCl | 83.9 (3.3) | A | ||||||
| Extraction | |||||||||
| 4 | None | Diethyl ether | 85.3 (1.9) | A | |||||
| 5 | Acid alcohol | None | 52.2 (7.2) | B | |||||
| 6, 7 | Acid alcohol | Diethyl ether | 85.7 (3.3) | 26.5 (18.4) | A | C | |||
| 8, 9 | Acid alcohol | Ethyl acetate | 82.4 (1.6) | 4.0 (6.1) | A | D | |||
| 10, 11 | Acetoacetic buffer | Diethyl ether | 84.5 (4.3) | 87.2 (2.1) | A | A | |||
| Incubation | |||||||||
| 12 | Distilled water | 72.8 (15.5) | A | ||||||
| 13 | 0.1 N H2SO4 | 82.4 (3.2) | A | ||||||
| 14 | 0.5% formalin | 82.7 (4.1) | A | ||||||
a
The value reported for percent viability is the mean of five replicate samples.
b
Values marked with the same letter are not significantly different with α = 0.05
c
30-min direct exposure to reagents. Rinsing steps decreased the residual ethanol concentration to approximately 2 g/liter during incubation.
d
1-h direct exposure to reagents. Samples were incubated with an approximate residual ethanol concentration of 102 g/liter.