TABLE 3.
A. aceti protein spots identified by microsequencing
| Proteins | Designation | Induction conditionsa | Inductionb | Size (kDa) | pI | N-terminal sequencec | Homologous proteins | Accession no. |
|---|---|---|---|---|---|---|---|---|
| Aaps | A | SC | + | 24 | 5.3 | DATPVPAPD . P | ||
| C | SC | + | 26 | 6.0 | . GQYEAIED | |||
| F | SC | + | 30 | 5.4 | AYITATDGTSLHVKD | Nonheme haloperoxidase (30 kDa) | O05691 | |
| G | SC | + | 33 | 5.5 | . . YPAPPFAT | |||
| I | SC | ++ | 39 | 6.3 | VNNQVKLQDD | Endonucleased | PC1254 | |
| J | SC | + | 39 | 6.5 | MQYRQLGRSG | Potassium channel β-subunit (31 kDa) | U46758 | |
| Oxidoreductase, aldo/keto reductase family (43 kDa) | E72284 | |||||||
| L | SC | ++ | 30 | 5.2 | ALTVLVTGAT | Putative hydroxylase (29 kDa) | AAF01810 | |
| Daunorubicin C-13 ketoreductase (31 kDa) | AAD04717 | |||||||
| Acetate-induced proteins in batch or continuous culture | 2 | S | ++ | 23 | 5.7 | . . RDRHVTQL . . NA | ||
| 3 | C | + | 23 | 5.1 | VKVAALATDGLEEIELT | Protease I (21 kDa) | AAF10772 | |
| GPQEAL . A | ||||||||
| 14 | S | + | 35 | 6.7 | PADPRTRYPR | |||
| 15 | S | + | 38 | 5.4 | MKTLLPTSTA | Pectate lyase II precursor (40 kDa) | P16530 | |
| 20 | C | ++ | 33 | 4.9 | MKTVTLRAGV | NADH:ubiquinone oxidoreductased | P25712 | |
| 23 | S | ++ | 49 | 5.7 | MKALTWQKKG | |||
| 24 | S | 47 | 5.6 | APVAFEHARL | 60-kDa chaperonin GroELd | P29134 | ||
| General stress proteins | 5 | CH | 17 | 5.9 | AEHVSGELKGTDGATHG | Superoxide dismutase (15 kDa) | AF021822 | |
| FVDVA . A | Putative aldehyde dehydrogenase | CAA19886 | ||||||
| 12 | O | 26 | 5.6 | AFELP . LAYA | Superoxide dismutase (∼22 kDa) | P81527 | ||
| 13 | SCH | 29 | 5.5 | MIGVPFPA . R | Putative thiol:disulfide interchange protein precursord | P52237 | ||
| 22 | SCH | 34 | 5.9 | MLIDIKNILL |
a
Induced under the following cultivation conditions: S, serial batch; C, continuous culture; O, osmotic stress; H, heat stress.
b
+, Two- to fourfold induction; ++, >4-fold induction.
c
Ambiguous residues are in italic. Dots, no amino acid identified.
d
Does not fulfill all significance criteria.