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. 2022 Jul 28;17(7):e0272231. doi: 10.1371/journal.pone.0272231

Fig 1. Effect of NAC on cell proliferation in C2C12 myoblasts.

Fig 1

Cells were exposed to 0–20 mM arecoline for 24 h (solid bars) or 48 h (open bars) (A) and 7 days (B). The viability of cells was measured with CellTiter 96 Aqueous One Solution Reagent (Promega) in duplicate. The means of duplicate from three independent experiments were used for further analysis. The values of ANOVA at 24 h, 48 h and 7 days were (F7,16 = 56.44, P = 4.28E-10), (F7,16 = 26.97, P = 1.03E-07) and (F5,12 = 7.93, P = 1.66E-3), respectively (mean ± SD; **p < 0.01, as compared to a no-treatment control, Tukey HSD test).