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. 2022 Jul 28;17(7):e0272231. doi: 10.1371/journal.pone.0272231

Fig 2. The effects of NAC on the decreased number of myotube formations caused by arecoline.

Fig 2

C2C12 cells were cultured in differentiation medium with 0–5 mM NAC and 0.04 mM (A, B) or 0.08 mM (C, D) arecoline for 7 days. The myotubes were photographed by phase contrast microscopy (A, C). Scale bar, 100 μm. The numbers of myotubes from 30 random fields were counted (B, D). The values of ANOVA with 0.04 and 0.08 mM were (F5,12 = 241.90, P = 1.30E-11) and (F5,12 = 379.27, P = 8.99E-13) (mean ± SD; **p < 0.01, as compared to cells that were treated only with arecoline, Tukey HSD test).