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. Author manuscript; available in PMC: 2022 Dec 24.
Published in final edited form as: Sci Immunol. 2022 Jun 24;7(72):eabo5407. doi: 10.1126/sciimmunol.abo5407

Figure 5. Intrinsic defects of FOXP3 ΔE2 Tregs in heterozygous Foxp3 exon 2 deletion female mice.

Figure 5.

(A) FOXP3 FL and FOXP3 ΔE2 Treg populations in heterozygous Foxp3 exon 2 deletion (ΔE2Het) female mice at the age of 2 months. Thym: thymus; pLN: peripheral lymph nodes; Spl: spleen; mLN: mesenteric lymph nodes; PP: Peyer’s Patches; IEL: intraepithelial lymphocytes of the gut; LPL: lamina propria lymphocytes of the gut. (B) Expression of FOXP3 in FOXP3 FL and FOXP3 ΔE2 Tregs in ΔE2Het female mice. (C) Mean fluorescent intensity (MFI) of phenotypic Treg markers CD25, CTLA-4, and GITR in FOXP3 FL and FOXP3 ΔE2 Tregs in ΔE2Het female mice. Grey line: FOXP3− cells; Red line: FOXP3 FL Tregs; Blue line: FOXP3 ΔE2 Tregs. (D) Flow cytometric analysis of CD44 and CD62L in Treg cells expressing either the FOXP3 FL (red) or FOXP3 ΔE2 (blue) isoform in pLN of ΔE2Het female mice. Data represent mean ± SEM from one of > 2 independent experiments.*: p < 0.05 **: p < 0.01; ***: p < 0.001; ****: p < 0.001 by paired two-tailed t-test.