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. Author manuscript; available in PMC: 2022 Dec 24.
Published in final edited form as: Sci Immunol. 2022 Jun 24;7(72):eabo5407. doi: 10.1126/sciimmunol.abo5407

Figure 6. Instability of FOXP3 ΔE2 Tregs.

Figure 6.

(A) Experimental design to examine stability of FOXP3 FL and FOXP3 ΔE2 Tregs. FOXP3 ΔE2 Tregs (Thy1.1+) and FOXP3 FL Tregs (Thy1.2+) were sort purified from mixed bone marrow chimeras. The Tregs were mixed at a 1:1 ratio, adoptively transferred (i.v.) into Tcrb deficient mice, and their identity was tracked by flow cytometry. (B) Percentage of Thy1.1+ (FOXP3 ΔE2 Treg derived) and Thy1.1− (FOXP3 FL Treg derived) donor cells in the blood over the 4 week period. (C) Percentage of Thy1.1+ (FOXP3 ΔE2 Treg derived) and Thy1.1− (FOXP3 FL Treg derived) donor cells still remaining FOXP3+ over time. (D) Flow cytometric analysis of FOXP3 expression in FOXP3 ΔE2 and FOXP3 FL Treg derived donor cells four weeks after transfer. (E) Tracking Treg stability using lineage tracing mice. Data represent mean ± SEM (n = 4 mice for B-D; and n = 3 mice for E) from one of 2 independent experiments. **: p < 0.01; ***: p < 0.001 by two-tailed t-test.