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Utilization of pyrene by Mycobacterium sp. strain AP1 in liquid mineral medium with pyrene as the sole source of carbon and energy at 25°C and 200 rpm. Growth is shown as an increase in the level of cell protein in cultures (▴) and in controls without a carbon source (▵). Pyrene concentrations were determined by HPLC analyses of organic extracts from cultures (●) and uninoculated controls (○).
