FIG. 3.

Immunodetection of the lipase in cellular extracts (A) and culture supernatant (B) of E. coli and P. fluorescens carrying different plasmids. E. coli DH5α and P. fluorescens SIK W1 harboring different plasmids were grown at different temperatures in LB medium. P. fluorescens did not grow at 37°C, so there was no result. The cells were harvested at the stationary phase when they reached an OD₆₀₀ of around 3.0. Cell extract and culture supernatant were prepared as described in Materials and Methods. Cell extract of 0.09 OD equivalent and culture supernatant of 0.04 OD equivalent were subjected to SDS-PAGE, transferred to nitrocellulose membrane, and analyzed by immunodetection. Lanes: 1, E. coli (pAJH5); 2, E. coli (pAJH10); 3, P. fluorescens (pAJH5); 4, P. fluorescens (pAJH10).