FIG. 6.

SDS-PAGE for culture supernatant of different culture conditions. The culture supernatants of three different conditions at three different times (23, 47, and 71 h) indicated by arrows in Fig. 5B were seen on SDS-PAGE. Each 16 μl of culture supernatant was subjected to SDS-PAGE. Lanes: SM, size marker; 1, well-aerated (100-ml baffle flask); 2, medium aeration (100-ml unbaffled flask); 3, microaerated conditions (350-ml unbaffled flask).