TABLE 1.
Microbial strains and plasmids used in this study
| Strain or plasmida | Relevant genotype | Source or reference |
|---|---|---|
| Yeast and fungal strains | ||
| S. cerevisiae Y294 | leu2-3,112 ura3-52 his3 trp1-299 | ATCC 201160 |
| S. cerevisiae Y294 | ||
| (fur1::LEU2 pDLG1) | bla URA3 ADH2PT | 24 |
| (fur1::LEU2 pDLG5) | bla URA3 ADH2P-xyn2-ADH2T | 24 |
| (fur1::LEU2 pDLG55) | bla URA3 ADH2P-MFα1S-xlnD-ADH2T | This work |
| (fur1::LEU2 pDLG56) | bla URA3 ADH2P-xyn2-ADH2TADH2P-MFα1S-xlnD-ADH2T | This work |
| T. reesei QM6a | ATCC 13631 | |
| A. niger | ATCC 90196 | |
| Bacterial strain | ||
| E. coli XL1-Blue MRF′ | Δ (mcrA)193 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac[F′ proAB lacIqZΔM15 Tn10 (Tetr)] | ZAP-cDNA synthesis kit (Stratagene) |
| Plasmids | ||
| pGEM-T Easy | bla | Promega |
| pRR1 | bla URA3 ADH2P-MFα1S-ADH2T | 37 |
| pDF1 | bla fur1::LEU2 | 24 |
a
S. cerevisiae Y294 (fur1::LEU2 pDLG1) is designated Y294 (VECT); S. cerevisiae Y294 (fur1::LEU2 pDLG5) is designated Y294 (XYN2); S. cerevisiae Y294 (fur1::LEU2 pDLG55) is designated Y294 (XLO2); S. cerevisiae Y294 (fur1::LEU2 pDLG56) is designated Y294 (XYN2 XLO2).