Skip to main content
. Author manuscript; available in PMC: 2022 Jul 28.
Published in final edited form as: Cell Host Microbe. 2021 Jun 29;29(8):1266–1276.e5. doi: 10.1016/j.chom.2021.05.009

Figure 3. VACV E3 Zα domain and dsRBD collaborate to regulate cell death.

Figure 3.

(A) Viability of L929 cells that were IFN-α-pretreated as described in Figure 1 and subsequently infected at an MOI of 5 with the indicated viruses. Cell viability was determined at 6 hpi by Sytox dye exclusion as described in Figure 1.

(B) IB of L929 cells that were IFN-α-pretreated as described in Figure 1 prior to infection at an MOI of 5 with the indicated viruses. Lysates were harvested at 4 hpi and, following SDS-PAGE, evaluated for phospho-MLKL.

(C) Viability of IFN-β-pretreated L929 cells were infected with a single virus at an MOI of 10 or coinfected with two viruses each at an MOI of 5. Cell viability was determined at 12 hpi by CellTiter-Glo assay as described in Figure 2.

(D) IB of IFN-β-primed L929 cells infected with single virus or coinfected with two viruses as described in (C). Lysates were harvested at 4 hpi and, following SDS-PAGE, evaluated for phospho-MLKL. Error bars represent the SD. Each set of data is representative of three replicates except for (A) and (C) which compiles the results of the replicates. Statistical significance was determined as described in Figure 1.